Journal: Journal of Animal Science and Technology

Article Title: Decoding the intestinal microbiota repertoire of sow and weaned pigs using culturomic and metagenomic approaches

doi: 10.5187/jast.2021.e124

Figure Lengend Snippet: (A) The summary of the culturomics work flow. Briefly, fresh fecal samples were transferred to an anaerobic chamber, serially diluted and plated on each medium. The cultured colonies were isolated. A pure culture was obtained through at least three subcultures, and the isolated bacteria were identified through 16S rRNA sequencing. (B) Molecular identification of culture-dependent isolates from fecal samples of sows or weaned pigs. Each pie chart displays all bacterial species isolated from sow and piglet fecal samples with microbial distribution. (C) Number of isolates isolated from several commercially available media. The number of isolates isolated from sow and piglet fecal samples was visualized as a heat map according to the corresponding isolated medium. PEA, phenylethyl alcohol agar; FAS, fastidious anaerobe agar; MRS, de Man Rogosa Sharpe agar; BS, Bifidobacterium selective agar.

Article Snippet: For molecular identification using 16S rRNA sequencing, universal primer set 27F-1492R was used to amplify the 16S rRNA gene fragments, and the amplified product was sequenced using an ABI 3730xl DNA analyzer (Applied Biosystems, Foster City, CA, USA).

Techniques: Cell Culture, Isolation, Sequencing